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KMID : 1171820080230040023
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Journal of Environmental and Sanitary Engineering
2008 Volume.23 No. 4 p.23 ~ p.29
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Mutagenesis of Streptomyces kasugaensis for Kasugamycin Production
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Cho Hoon
Choi Du-Bok
Lim Chae-Kyu
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Abstract
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This study was performed to develop mutant strain using a combination of UV irradiation procedures with protoplast mutagenesis in order to achieve an effective kasugarnycin production from Streptomyceskasugaensis. Whenlessthan1.0g/lofLinoleic acid was used, the cell growth was not inhibited. On the other hand, the cell growth was greatly inhibited when more than 1.6 gil of linoleic acid was used. Among the various mutant strains, SK-12 was obtained in medium containing 1.6g/l of linoleic acid, showing the highest rate of both cell growth and kasugarnycin production. In order to compare kasugarnycin production with the SK-12 and the parent strain using soybean oil, cultures were performed in a flask. The production of kasugamycin was increased with the increase time. The maximum kasugamycin concentration was 1.2g/l after 6 days of culture. The product yield from soybean oil was 0.05g/l/g consumed carbon source, which was roughly 5.0 fold higher than the parent strain. These results show that it was effective method for obtaining a mutant resistant to linoleic acid for the effective production of kasugamycin from soybean oil.
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KEYWORD
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kasugamycin, Streptomyces kasugaensis, Linoleic acid
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